vgulati at lgt.org.uk wrote:
: Hi,
: I am a PhD student at St. Mary's Hospital and am trying to make
: Tcell lines for my project on hepatitis. I have references which
: say that adding lymphocult T to my lines can be helpful (as a
: nonspecific stimulant) I havnt been able to find a UK supplier of
: this product.
You may contact Biotest Diagnostic, Dreieich, Germany. There should be
some supplier in UK. Couple of years ago i successfully used this to
develop several T cell lines. If not this, you may try using recombinant IL-2
at final concentration of 10U/ml.
Raju
