You may try coating test plates with biotinylated antigens followed by
back-coating with some blocking buffer (e.g. 1% BSA plus Tween 20).
Then add an optimized concentration of strepavidin-HRP to generate
a readable signal(OD 1.0-2.0) when substrate added. This will not give
you an absolute amount of bound antigen but a fairly reasonable
comparison among three antigens you anticipate to use. You should also
determine biotin/collagen molar ratios to determine how similar
the capacities of these three antigens to bind strepavidin-HRP are.
Good luck.
Z. Altin
Antibody Concepts
zafer at abconcepts.com
web site:http://www.abconcepts.com
