I am wanting to make fab fragments from a rat IgG2a that was purified
from a serum free hybridoma supe. Pierce Chemical sells immobilized
pappain, but I am afraid the fab fragment after digestion will be
difficult to purify via Protein G. If any of you could send me a
reference for making fabs from rat IgG2a I would greatly appreciate your
help. The only reference I have is from 1983 (before the advent of
Protein G) My E-mail address is rjjensen at inav.net Thanks for your help.
