------- Forwarded Message Follows -------
From: "Alicia Fernandez" <ALICIA at ict.sld.cu>
Organization: Centro de Inmunologia Molecular
To: admin at ict.sld.cu
Date sent: Tue, 26 Nov 1996 17:26:08 EDT
Subject: Re: Ciprofloxacin treatment
Priority: normal
Kiley R. Prilliman wrote:
Could anyone give me any information regarding cleaning up
mycoplasma-contaminated cell cultures using ciprofloxacin? My cultures
are of B lymphoblastoid cell lines, and I'm intending to use 10 g/mL
ciprofloxacin as treatment...does anyone know how effective this
protocol is, and how well my cells might survive such treatment?
Your assistance in this matter is sincerely appreciated!
Kiley R. Prilliman
Dept. of Microbiology & Immunology
OUHSC
==================================
The answer is the following: usual concentration for treatment is 10
ug/ml and the original report about ciprofloxacin treatment is in
Journal of Immunological Methods, 109, 17:25, 1988, Schmitt et al.
We have used this antimycoplasma antibiotic for our hybridomas
strains and is very useful.
My address is: alicia at ict.sld.cu
__
Alicia Fernandez Ordonez
Division de Desarrollo
Centro de Inmunologia Molecular
La Habana
Cuba
email: alicia at ict.sld.cu
