Try using an EDTA-PBS solution. Then gently blow off the cells with a
pipet. Generally, macrophages, lymphocytes, etc. will tend to shed their
receptors even under this condition when released from tissue culture
plastic. Unfortunately this is the nature of these cells.
In article <32510893.2B64 at nimr.mrc.ac.uk>, anybody
<anybody at nimr.mrc.ac.uk> wrote:
> I am looking at apoptosis in infected macrophages, this is an 'in vitro'
> study. The problem is this : How do I remove them from the culture
> vessel without losing the cell surfaces markers? I will require to know
> the cell type of the apoptotic cells.
>> Best
>>b-butler at nimr.mrc.ac.uk
