Hello,
I am currently a grad student at the University of Georgia, and am
planning on doing some RT-PCR experiments for RAG expression. I'd like
to use primers which span an intron, so that I can differentate RAG mRNA
signals from signals generated from contaminant genomic DNA. The problem
is, I cannot for the life of me find the genomic sequences for RAG1 and
RAG2 in mice. I have found genomic sequences for over 10 species, but
not mice. I'm beginning to think
that this sequence has not been done in mice yet. Does anyone know? I
have gotten the cDNA sequences for these genes in mice, and I've read 6-7
papers about the RAG locus, but I can't seem to locate this information.
I've also done computer searches for just about every permutation of RAG
(and recombinase/ation activating/associated gene, etc etc).
If anyone has this info, or knows of a way of obtaining it, please email
me (jreese at cellmate.cb.uga.edu)
Thanks in advance,
Justin Reese
University of Georgia
Department of Cellular Biology
jreese at cellmate.cb.uga.edu
