In article <54qk86$am at netman-mel.dfci.harvard.edu>,
mohan_natesan at dfci.harvard.edu (Mohan) writes:
> 1. Can someone suggest a method which will yield respectable yields?
Mohan, why don't you try bone-marrow derived DCs? Easily cultured in large
numbers. Look at Mayordomo JI et al, Nature Medicine. 1(12):1297-302, 1995
for methods and references.
> 2. Also how many DC needed to be added to see some IL-2/IFN production
> by T cells (for 1x10 5 T cells) ?
It depends on your experimental situation. Be aware that adding too many
DCs is A Bad Thing and will decrease readouts such as MLR. I wouldn't go
above a DC:T cell ratio of 1:4 at the most.
Hope this helps.
Ian Davis iddavis at vms.cis.pitt.edu
University of Pittsburgh Cancer Institute
