IUBio

Effect of Tween?

Jim Koch "jkoch at jkoch" at std.tdh.state.tx.us
Thu Apr 3 17:12:35 EST 1997


Jared Head wrote:
> 
> Here's another question about my Alkaline Phosphatse ELISA.
> 
> I've just run parallel ELISA plates binding an AP-conjugated antibody to
> its epitope bound to the plate.  The only difference between the two
> plates was that in one I bound and then washed the antibody-conjugate with
> PBS, in the other I used PBS/0.1% Tween.
> 
> I was surprised to see that I got strong binding in the case of the tween,
> but no binding at all with straight PBS.  If anything I would have
> expected higher binding *without* the tween due to increased non-specific
> binding.  Can anybody explain why tween has increased the binding, and
> ease my confusion?
> 
> Cheers,
> 
> Jared
> 
> --
> Jared Head     at the Department of Biochemistry, University of Bristol
> 
> "A computer lets you make more mistakes faster than any invention in human
>      history - with the possible exceptions of handguns and tequila."
>                                                    Mitch Ratliffe
tween acts as a surfactant and probably cleans some of the lipid soluble
material prior to the addition of the Ig-congugate, allowing better
binding of the Ig
jim



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