If you are preparing your glass slides in a glass container you
may find that your slides are not adhering properly because you coated
your container also. I find that the positively charged slides sold by
Fisher Scientific work much better than those coated with APTEX or
Poly-L-lysine. I rarely lose tissue this way and I use to routinely lose
tissue by coating my own slides. These "plus" slides are a bit more
expensive than regular slides but I think they are worth every penny by
saving wasted time and samples.
Betty-Anne