What is the nature of your assay?
Jared Head (bijgh at zeus.bris.ac.uk) wrote:
: Here's another question about my Alkaline Phosphatse ELISA.
: I've just run parallel ELISA plates binding an AP-conjugated antibody to
: its epitope bound to the plate. The only difference between the two
: plates was that in one I bound and then washed the antibody-conjugate with
: PBS, in the other I used PBS/0.1% Tween.
: I was surprised to see that I got strong binding in the case of the tween,
: but no binding at all with straight PBS. If anything I would have
: expected higher binding *without* the tween due to increased non-specific
: binding. Can anybody explain why tween has increased the binding, and
: ease my confusion?
: Cheers,
: Jared
: --
: Jared Head at the Department of Biochemistry, University of Bristol
: "A computer lets you make more mistakes faster than any invention in human
: history - with the possible exceptions of handguns and tequila."
: Mitch Ratliffe
