In article Grace Ho writes:
> Anyway, I have read somewhere that T- cells do not really
>proliferate, that is why the MTT is not being taken up and
>converted....
Grace,
MTT is converted by enzymes in active mitochondria to the water-insoluble
product formazan. The conversion in dependent on active mitochondria, thus on
living cells, but NOT on proliferation of the cells. MTT should thus also be
converted by non-proliferating cells. The tradiational thymidine
incorporation assay is dependent on DNA construction, and for this reason only
labels proliferating cells.
Sorry, but I don't have any ideas about why your cells are not converting MTT.
Good luck to you!
linda