IUBio

Expression Cloning strategies for finding microbial CD8 antigens

Ken Frauwirth BioKen frauwirt at bacillus.Berkeley.EDU
Thu Apr 24 19:43:38 EST 1997


In article <Pine.A41.3.95b.970423222238.22428A-100000 at homer34.u.washington.edu>,
David Koelle  <viralimm at u.washington.edu> wrote:
>Any words of wisdom or literature references?  I am familiar with the
>trail that led to MAGE antigens.  Anything new with making libraries in
>mammalian expression vectors, vaccinia, retroviruses, etc. to perhaps sort
>out CD8 antigens in a moderately large virus?  Maybe some people have
>tried and failed or succeeded witht he published approaches.  I am more
>interested in a direct approach than peptide pool sequencing or predicting
>from motifs when the HLA restricting allele is known.

The Shastri Lab has been interested in the expression cloning of T cell 
antigens for a number of years.  Members of the lab have successfully
identified both the genes and specific peptides for CD4+ and CD8+ T cells
using bacterial and mammalian libraries.  I refer you to several papers 
regarding the identification of Class I restricted antigens:

Malarkannan, S, Afkarian, M, and Shastri, N.  A rare cryptic translation 
product is presented by K(b) MHC class I molecule to alloreactive T-cells. 
J Exp Med 182:1739-1750

Malarkannan, S, Serwold, T, Nguyen, V, Sherman, L, and Shastri, N.  The MMTV 
env gene is the source of a CD8+ T-cell stimulating peptide/MHC class I 
complex in a murine thymoma.  PNAS 93:13991-13996

Malarkannan, S, Gonzalez, F, Nguyen, V, Adair, G, and Shastri, N.  Allo-
reactive T-cells can recognize unusual, rare, and unique processed peptide/
MHC complexes.  J Immunology 157:4464-4473

Shastri, N.  Needles in Haystacks: Isolating peptide antigens for specific
T-cells.  Curr Op in Immunology 8:271-277


Ken Frauwirth
-- 
Ken Frauwirth (MiSTie #33025)                         _           _
frauwirt at mendel.berkeley.edu                         |_) *    |/ (_ |\ |
http://www.ocf.berkeley.edu/~frauwirt/               |_) | () |\ (_ | \|  
DNRC Title: Chairman of Joint Commission on In-duh-vidual Affairs



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