IUBio

neutrophil separation

Graham Briars g.briars at mailbox.uq.edu.au
Mon Apr 28 02:54:46 EST 1997


While  we are on the subject. I have been using the Ficoll Hypaque 
modification of Ferrante and Thongs method. I regularly get
half of the red cells appearing at the bottom of the tube and half
suspended about 1mm below the neutrophil layer. It makes pipetting the
neutrophils a bit more difficult, but it works. Has anyone else had and
resolved this problem.



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