I'm considering of using the pHOOK vectors of invitrogen for transfection
experiments with T lymphocytes. Transfectants containing the pHOOK vectors
can be seperated by using magnetic beads and a magnete.
My questions:
1. Does anyone have experience with this technique??
2. What cellines have you used for this technique??
If someone has any tips please reply
Thanks a lot
please reply to : hellings at luc.ac.be
--
Niels Hellings, PhD student
Ms research Unit Immunology research group
Dr. L. Willems-Institute Dept. of Physiology, LUC
University Campus University Campus
B-3590 Diepenbeek B-3590 Diepenbeek
Belgium Belgium
Voice ++32(0)11/26.92.07
Fax ++32(0)11/26.92.09