I've been trying to work with thymocytes in vitro. I would just
like to be able to culture them for 24-48 hours without losing
too much viability as I am trying to induce apoptosis. However,
every time I culture thymocytes they rapidly die (about 50% just
overnite). Can anyone offer some tips on how to keep the thymocyte
viability at a reasonable level (at least better than 75%) after
a 24 hr + culture period. They are now just in DME 10%FCS 10%CO2
2ME 5E-5M.
Thanks
Rick
Boston U
rocket1 at bu.edu
