I am trying to standardize a PCR method for Bcl1 rearrangement for mantle
cell lymphoma. I am using primers used by several groups and published
but am unable to reproduce results. I have done the usual Mg++
concentration titration and different annealing temperatures.
Is there anyone who has had to standardize this particular rearrangement
technique who can give me some hints or is willing to dialogue with me. I
need help!
