Hi all,
We have an IgM antibody which is difficult to purify from ascites!
The problem is that it falls out of solution very easily. Have tried
peg purification which usually is an effective method (for IgM's) but
the final pellet wouldn't go back into solution ( in carbonate
buffer). So I had to use a tris buffer to get it into solution.
I then passed it through a column (equilibrated with Carb-buffer) but
lost a lot of antibody in this step! and then conjugated what
antibody was in solution. The trouble is that this conjugate is
now precipitating out! Any suggestions
Chris Weir
Macquarie University
Sydney Australia
