Hi all,
I have been trying to stain fixed/permeabilized cells grown on coverslips
with IgM mouse monoclonal antibodies. I use biotinylated horse anti-mouse
followed by streptavidin-CY3. I have tried a number of dilutions of all
the reagents involved but I get background - red specks all over the cells.
I have tried different permeabilization and fixation methods. Nothing
seems to help.
I do not know what to do next. I would appreciate any suggestions.
Thanks
Gabriel
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Dr Gabriel Kremmidiotis E-mail:gkremmid at mad.adelaide.edu.au
Centre for Medical Genetics
Women's and Children's Hospital Telephone
72 King William Road (National): (08)8204 6270
North Adelaide (International):(61-8)8204 7342
SA 5006
Australia Fax: 8204 7342
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