Department of Molecular Biology and Genetics
Johns Hopkins University School of Medicine
Ross Research Bldg., Rm. 1050
720 N. Rutland Ave.
Baltimore, MD 21205
January 5th, 1997
To whom it may concern:
I am writing in regards to potential post-doctoral / entry level positions
that may be available in the areas of basic science AIDS research and HIV
vaccine development.
I am a 5th year Ph.D. candidate in the Biochemistry, Cellular and Molecular
Biology Training Program at the Johns Hopkins University School of Medicine. I
plan to complete my graduate program in the spring/summer of 1997.
My research, under the direction of Robert F. Siliciano M.D. Ph.D., has been
focused in two areas related to HIV/AIDS research. I developed a novel method
(AIDS Research and Human Retroviruses, 10: 1423, 1994) for the detection
and evaluation of cytolytic T-cell responses to candidate AIDS vaccines
being tested
in Phase I clinical trials at the Center for Immunization Research here at
Johns Hopkins (J. Infect. Dis. 171: 1623, 1995). This method is now widely
used in the testing of AIDS vaccines.
More recently my thesis work has focused on developing a better understanding
of the role of a highly conserved YXXL internalization motif within the
envelope glycoprotein gp41 of HIV-1 in viral assembly, host immune recognition,
and CD4 cell loss (J. Virol., 10:6547-6556 (1996)).
I seek a post doctoral / entry level position in the area of basic
sciences AIDS research and HIV vaccine development. My nine years research
experience, my excellent writing and verbal skills, along with my proven
ability to publish in peer reviewed journals makes me an excellent
candidate.
I welcome an opportunity to discuss my capabilities with respect to
opportunities that may exist in this area. I have enclosed a copy of my
curriculum vitae along with the name and addresses of three references for
your information.
Please feel free to contact me by mail at the above address or by telephone or
E-mail:
Phone: 410-955-7757
Fax: 410-955-0964
email: egan at welchlink.welch.jhu.eduhttp://www.welch.jhu.edu/~egan
Sincerely,
Michael A. Egan
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CURRICULUM VITAE
Name: Michael A. Egan
Current Appointment: Ph.D. Candidate, Graduate Program in
Biochemistry, Cellular and Molecular Biology,
Johns Hopkins University, School of
Medicine,
Baltimore, MD
Office Address: Johns Hopkins University School of Medicine,
Department of Medicine
Rm. 1050 Ross Research Bldg.
720 N. Rutland Ave.
Baltimore, MD 21205
Phone: 410-955-7757
Fax: 410-955-0964
e-mail: egan at welchlink.welch.jhu.eduhttp://www.welch.jhu.edu/~egan
Home Address: 3721 Rexmere Rd.
Baltimore, MD 21218
Phone 410-366-3268
EDUCATION
1992-present Ph.D. Candidate, Graduate Program in
Biochemistry, Cellular and Molecular Biology
(BCMB), Johns Hopkins University, School of
Medicine, Baltimore, MD
1987-1992 B.S., Chemistry, Magna Cum Laude,
Northern Arizona University, Flagstaff, AZ
HONORS AND AWARDS
Undergraduate Magna Cum Laude in Chemistry
Education Grade Point Average 3.80/4.00
NSF Summer Research Fellowship
Golden Key National Honor Society
Phi Lambda Upsilon Chemistry Honor Society
Phi Kappa Phi Honor Society
Dean's List seven semesters
Post Graduate Fellowship, Graduate Program in Biochemistry,
Education Cellular and Molecular Biology (BCMB), Johns
Hopkins University School of Medicine
WORK EXPERIENCE
1988-1991 Undergraduate Chemistry lab teaching assistant,
Northern Arizona University
Summer 1991 NSF Research Fellowship, University of Georgia
1983-1987 Enlistment in the U.S. Marine Corps, honorable
discharge.
RESEARCH EXPERIENCE
1992-Present Elucidating the role of the YXXL
internalization
motif within the envelope protein (gp41) of
HIV-1 in viral assembly, immune
recognition, and
CD4 cell loss. Developed a novel method,
now
widely used in the AIDS field, for the
detection
of (HIV-1)-specific CTL responses in human
vaccine recipients. In a Phase I
clinical
trial evaluated the ability of an experimental
HIV-1 vaccine to elicit HIV-specific CTL
responses in sero-negative volunteers.
Laboratory of Robert F. Siliciano,
Department of Medicine,
Johns Hopkins University School of Medicine.
1991-1992 Using semi-empirical computational methods to
calculate the structure and electronic
properties of the mono-boro-analogs of
acetylcholine.
Laboratory of Dr. Robert W. Zoellner,
Department of Chemistry,
Northern Arizona University
NSF 1991 Summer Mutagenesis of murine ferrochelatase in looking
Fellowship for molecular mimicry in erythropoietic
protoporphyria.
Laboratory of Dr. Harry A. Dailey, Department of
Microbiology, University of Georgia
1988-1992 The effect of endurance training on glucose
metabolism.
Laboratory of Dr. Scott Savage and Dr.
Hans M.
Gunderson, Exercise Physiology Lab, Northern
Arizona University
SKILLS
Trained in standard molecular biology techniques, PCR, SDS-PAGE, Western
Blot, Pulse Chase Analysis, the use of Fluorescence Activated Cell Sorter
analysis (FACS), all aspects of human tissue culture, and optimization and
implementation of standard immunological assays (Cr51 release, CTL assays,
ADCC, ELISA). Five years experience in the handling and use of high titer
infectious material (HIV-1, vaccinia virus, EBV) under Biohazard Level 3
(P3) laboratory conditions. Computer trained with experience programming
in FORTRAN, C/C++, HTML. Skilled in the use of DOS/Windows, Mac and Unix
operating systems. Able to efficiently access and publish information on
the WWW and Internet.
PUBLICATIONS
1. Michael A. Egan and Robert W. Zoellner. Structural and Electronic
Characteristics of the Monoboro-Analogs of the Acetylcholine Cation As
Determined by the Semiempirical MNDO Computational Method. J. Org. Chem.,
58:1719-1729 (1993).
2. M.N. Lubaki, Michael. A. Egan, Robert. F. Siliciano, Kent. J. Wienhold,
and Robert. C. Bollinger. A Novel Method for the Detection and Expansion
of HIV Type 1-Specific Cytolytic T Lymphocytes. AIDS Res. Hum.
Retroviruses, 10:1423-1427 (1994).
3. Michael A. Egan, Wendy A. Pavlat, James Tartaglia, Enzo Paoletti, K. J.
Wienhold, Mary Lou Clements, and Robert F. Siliciano. Induction of Human
Immunodeficiency Virus Type 1 (HIV-1)-Specific Cytolytic T Lymphocyte
Responses in Seronegative Adults by a Nonreplicating,
Host-Range-Restricted Canarypox Vector (ALVAC) Carrying the HIV-1MN env
Gene, J. Infect. Dis., 171:1623-1627 (1995).
4. Robert. C. Bollinger, Michael. A. Egan, Tae-Wook Chun, Bonnie
Mathieson, and Robert F. Siliciano. Cellular Immune Responses to HIV-1 in
Progressive and Non-Progressive Infections. AIDS, 10:1-12 (1996).
5. Michael A. Egan, Lucy M. Carruth, Jennifer F. Rowell, Xiaofang Yu, and
Robert F. Siliciano. HIV-1 Envelope Protein Endocytosis Mediated by a
Highly Conserved Intrinsic Internalization Signal in the Cytoplasmic
Domain of gp41 Is Suppressed in the Presence of the Pr55gag Precursor
Protein, J. Virol., 70:6547-6556 (1996).
Abstracts
1. Michael A. Egan and Robert W. Zoellner. The Mono-Boro-Analogs of
Acetylcholine: Structural and Electronic Comparisons to the Parent
Acetylcholine Using the MNDO Computational Method. Annual American
Chemical Society Meeting, San Francisco, CA, April 1992.
2. Michael A. Egan, Kent Weinhold, Enzo Paoletti, James Tartaglia, Mary
Lou Clements, Wendy Pavlat, and Robert F. Siliciano. Cytotoxic T
Lymphocyte Responses to Recombinant Canarypox-gp160 MN (ALVAC vCP125,
HIV-1 gp160 MN) in HIV-1 Uninfected Adult Volunteers. The 6th Annual
Conference on Advances in AIDS Vaccine Development, Alexandria, VA,
October 30th - November 4th, 1993.
3. Michael A. Egan, Wendy Pavlat, James Tartaglia, Enzo Paoletti, Robert
F. Siliciano and Mary Lou Clements. Induction of HIV-1-specific Cytotoxic
T Lymphocyte Responses in Seronegative Adults by a Non-Replicating, Host
Range Restricted Recombinant Canarypox Vector
Carrying the HIV-1 env Gene. The 7th Annual Conference on Advances in
AIDS Vaccine Development, Reston VA, November 6th-10th, 1994.
4. Michael A. Egan, Wedy Pavlat, James Tartaglia, Enzo Paoletti, Mary Lou
Clements and Robert F. Siliciano. Cytotoxic T Lymphocyte Responses to
Recombinant Canarypox-gp160 MN (ALVAC vCP125, HIV-1 gp160 MN) in HIV-1
Uninfected Adult Volunteers. The Frank B. Polk Symposium on AIDS,
Baltimore, MD, April 15th-16th, 1994.
5. Michael A. Egan, Jennifer F. Rowell, Robert F. Siliciano and Lucy M.
Carruth. The Role of the gp41 (YXXL) Internalization Motif in HIV-1
Assembly.", Third Conference on Retroviruses and Opportunistic Infections,
Washington, DC, January 28th - February 2nd, 1996.
REFERENCES
Robert F. Siliciano, M.D., Ph.D.
Assistant Professor, Department of Immunology
Johns Hopkins University School of Medicine
Rm. 1049, Ross Research Bldg.
720 N. Rutland Ave.
Baltimore, MD 21205
Office: 410-955-2958
Fax: 410-955-0964
e-mail: rsilicia at welchlink.welch.jhu.edu
Robert C. Bollinger, M.D., M.P.H.
Assistant Professor, Division of Infectious Disease
Johns Hopkins University School of Medicine
Rm. 1159, Ross Research Bldg.
720 N. Rutland Ave.
Baltimore, MD 21205
Office: 410-614-0936
Fax: 410-955-7889
e-mail: rcb at welchlink.welch.jhu.edu
Xiao-Fang Yu, M.D., Ph.D.
Department of Molecular Biology and Immunology
Johns Hopkins University
School of Hygiene and Public Health
615 N. Wolfe, St.
Baltimore, MD 21205
Phone: 410-955-3768
Fax: 410-955-0105
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Michael A. Egan
Department of Molecular Biology and Genetics
Ross Research Bldg., Rm. 1050
Johns Hopkins University
720 N. Rutland Ave.
Baltimore MD 21205
Phone: 410-955-7757
Fax: 410-955-0964
email: egan at welchlink.welch.jhu.eduhttp://www.welch.jhu.edu/~egan
