Patti W. Jareo wrote:
>> Both Sigma and Gibco BRL have media that would be worth your time to
> look into. Growth is slower in these media, but the cells do appear to
> be healthy and produce antibodies just fine.
>> Good luck.
I have found GIBCO's Hybridoma SFM to be an excellent serum-free medium.
However, it does contain protein. I also find that with this medium
and most other serum-free media for hybridomas the cells die quickly
when they reach high densities and should be split back regularly. I
usually add 0.1% FBS to serum-free medium and the cells seem to grow
faster and appear more robust.
Sigma has a serum-free hybridoma medium that is also protein-free. It
is quite good, but the adaptation of the cells from "regular" medium
requires more time and greater care.
Rick Schuman
