To anyone out there who has measured intracellular calcium flux by flow
cytometry!
I need to measure intracellular calcium flux following crosslinking of
cell surface molecules. Because of the antibodies available in the lab, I
have to use a purified antibody as the staining reagent, and an
anti-antibody as the crosslinking reagent.
If you have a protocol for this kind of thing, or any ideas, please let
me know!
Thanks!
Seb :)
trops at ircm.umontreal.ca
