A.Marshall1 at shef.ac.uk (A Marshall) wrote:
>Please could anyone tell me what is the best way to purify mouse Fab
>proteins. The Fab is being expressed in a bacterial system. I have
>tried anti-IgG-agarose affinity purification but this was unsuccessful
>and am about to try Protein G.
>Any advice would be appreciated.
>Thanks.
Save your time/money, the Protein G is also unlikely to work it binds
to the Fc region of the intact antibody (which you don't have).
Was the anti-IgG heavy or light chain specific? You must use
antibodies which have anti-light chain x-reactivities - for the same
reason as above.
Also, did you check that your anti-IgG affinity column could actually
purify intact mouse IgG?
Pete
