I am having the problem that I want to use a mouse antibody on mouse
cryostatsections. So I have to come back with a anti-mouse PO.
Are there any ways to overcome the background this will give????
Please e-mail me your suggestions.
Menno de Winther mennow at ruly46.medfac.leidenuniv.nl
Dear menno de Winther:
The background was usually caused by the Fc receptor on mouse cell. so if you
use a mouse antibody on mouse cryostatsections, the antibody will bind to
cryostatsections by Fab to the antigen or by Fc to Fc receptor. I do not know
what kind of antibody you used, perhaps you could try to:
1.block the Fc receptor by rabbit serum if the background was not caused by B
cells on the cryostatsections (because B cells have membrane Ig). and
2.label your antibody directly with enzyme.
I hope these will improve it a lot.
wish you good luck in your experiment.
Sun Kai
e-mail:sunkdym at public.xa.sn.cn
Depert. of Immunology
The fourth Military Medical University
Xi'an, 710032
PR China
