Yiu-Loon Chui wrote:
>> Can anyone out there kindly update me on the current advance in HLA
> typing, especially for HLA-A, B and C? Is serotyping still the main
> methodology? How about typing by PCR? Are there PCR primers available?
> How reliable are they?
>> Thanks!
>> Yiu-Loon Chui
> Associate Professor
I'm performing HLA Class I (ABC) DNA typing by ARMS-PCR SSP and
PCR-SSOP.
Sure I find those techniques reliable.
There several papers on it, start by this one:
Phototyping: comprehensive DNA typing for HLA-A, B, C, DRB1, DRB3, DRB4,
DRB5, & DQB1 by PCR with 144 primer mixes utilising sequence-specific
primers (PCR-SSP)
M Bunce et al.
Tissue Antigens 1995, 46:355-367.
Feel free to ask for help.
--
Paulo Santos
Centro de Histocompatibilidade do Centro - LUSOTRANSPLANTE
Faculdade de Medicina de Coimbra Fax +351-39-33674
3º piso Apartado 3004 Telephone +351-39-33693
P-3049 COIMBRA Codex PORTUGAL +351-39-20527
