Jim Wu wrote:
>> I am looking for PDGF antibodies, preferably reactive against native,
> human material, which I assume must cover all of the isoforms: AA,
> AB, and BB. In addition, it is important that this antibody can be
> used for:
>> 1) neutalising activity in proliferative assays
>> 2) coupling to a column gel for removing PDGF from cell supernatants
>> I have tried R&D Systems polyclonal #AB-23-NA in my bioassay, and it
> did not appear to neutralise activity under my particular conditions
> at a concentration of 10ug/ml (ab was preincubated with std for 2 hrs
> and compared to an IgG control). It does work well, however, in
> Western blots.
>> Please email me, if you have any suggestions.
>> thanks in advance,
>>jim_wu at berlex.com
These researchers used a MAb against the B subunit. You might get in
contact with
them.
--
Jeffrey Smoot
Chemical Abstract Services Phone 614-447-3600 X3198 E-mail:
jsmoot at cas.org
P.O. Box 3012 WWW http://www.cas.orghttp://www.ChemCenter.org
Columbus, OH 43210 http://stneasy.cas.org
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TI Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB
binding to .alpha. and .beta. PDGF receptor
AU Fretto, Larry J.; Snape, Andrea J.; Tomlinson, James E.; Seroogy,
Joseph J.; Wolf, David L.; LaRochelle, William J.; Giese, Neill A.
CS COR Therapeut. Inc., South San Francisco, CA, 94080, USA
SO J. Biol. Chem. (1993), 268(5), 3625-31
