I am looking for PDGF antibodies, preferably reactive against native,
human material, which I assume must cover all of the isoforms: AA,
AB, and BB. In addition, it is important that this antibody can be
used for:
1) neutalising activity in proliferative assays
2) coupling to a column gel for removing PDGF from cell supernatants
I have tried R&D Systems polyclonal #AB-23-NA in my bioassay, and it
did not appear to neutralise activity under my particular conditions
at a concentration of 10ug/ml (ab was preincubated with std for 2 hrs
and compared to an IgG control). It does work well, however, in
Western blots.
Please email me, if you have any suggestions.
thanks in advance,
jim_wu at berlex.com
