IUBio

Homemade Chemiluminescent Substrates?

Dr E. Buxbaum EB15 at le.ac.uk
Mon Jun 2 08:07:54 EST 1997


Keld Sorensen <KeldS at uic.edu> wrote:

>Say you use 10 ml of a 1:500 dilution of a primary antibody that cost
>you $1,000/mg, then the antibody cost is $20 per blot. If you had to use
>say a 1:250 dilution (weak affinity antibody) then the cost would be $40
>per blot!!

This antibody solution would be reused many, many times. The costs per 
blot are, therefore, much lower. 




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