In article <338E908D.C9E at alpha2.curtin.edu.au>, Grace Ho
<EHO17 at alpha2.curtin.edu.au> wrote:
> Hello everyone,
> I am pretty confuse here about the route of immunisation!!! I am
> currently working with a mouse model where I take my protein antigen in
> Freund's adjuvant.... and immunised the victim via intraperitoneal route
> .... in the hope of stimulating a good systemic response as well as good
> T-cells reponse....
> However, I have been receiving advice from people (immunologists)
> and found that ip. immunisation is not a good way to go about if I want
> a good systemic/T-cells reponse..... They all recommended subcutaneous
> injection or intradermal injection....
> Problem is I am so used to ip. route immunisation and my work have
> been sort of based on ip. immunisation.... and things have not been
> working very well for me... and on the other hand, if I change the route
> of immunisation.. then my work is sort of down the drain!!!!
> Does anyone have any insight to this!!!!????? Please!!!??
>
Hello again Grace,
there is a little insight available on this subject, yes. The route of
immunisation is equally important in obtaining a 'good' immune respone as
the choise of antigen & adjuvants.
Apparently, the topological route of entry selects for a specific subset
of antigen presenting cell. In other words; ip/freunds will preferentially
use the intraperitoneal cavity macrophages as APC's, while the intrademal
route will locate the antigen near the Langerhals cells there. These will
take up the antigen and migrate to the draining lymph nodes, maturing to
dendritic cells there. Both APC-derived cytokines and adhesion molecules
on the APC seem to make a difference in the resulting response.
The type of antigen presenting cell influences the response you'll get;
therefore, route of entry will -indirectly- influence the response as
well.
Sorry to say , but your results obtained by ip immunisation will be hard
to match with the ones obtained through id. However, you might make a
point in your project by comparing the results of both methods.
Best of luck,
Guy
--
Guy Hermans, PhD student
Ms research Unit Immunology research group
Dr. L. Willems-Institute Dept. of Physiology, LUC
University Campus University Campus
B-3590 Diepenbeek B-3590 Diepenbeek
Belgium Belgium
Voice ++32(0)11/26.92.07
Fax ++32(0)11/26.92.09
