I am trying to express beta-gal in lymphocytes. I am using a plasmid
that contains the beta-gal gene attached to CMV promotor and this
plasmid was electroporated into a T-lymphocytes cell line (JJhan). This
plasmid was expressed efficiently in non-lymphoid cell line. I could not
detect any beta-gal activity in the T-lymphocytes using the X-gal
staining method.
Does any one know about any successful X-gal assay protocol for
lymphocytes or any reason why this assay is not working in lymphocytes?
I must say that we are using electroporation (gene pulser) routinely to
transfect T-lymphocytes with plasmids and transfected plasmid DNA can
be easily detected in southern blott.
Thanks,
Oded
