We are trying to repeat some old neutrophil chemotaxis experiments with
neutrophils and macrophages responding to fMLP. We have isolated mouse
peritoneal cells before and after thioglycolate injection adn then
simply looked for random motility stimulated by uniform fMLP in buffer
or media. The cells come out very round and rarely attach to glass or
plastic, spread or move before or after fMLP addition. Is there some
trick we are missing here that gives activatable cells? Thanks, Dave
