Pete (bss3pk at surrey.ac.uk) wrote:
: In the absence of other information, the answer must be, probably.
: Additionally, and this is especially true of ELISA's, don't begin to
: panic until, checked what you physically did, and then you've repeated
: the effect
:: a) with the same reagents
: b) newly made-up reagents
Thanks!
: BTW we have switched to storing our TMB stock diluted in DMF rather
: than DMSO, because its still liquid at 4 deg C (sometimes every minute
: counts).
Yes, it's a bit of a pain remembering to take the DMSO out of the fridge
in time. Presumably other than that, there isn't any difference between
the two solvents for this procedure? Whatever, it sounds like a sensible
idea to swap to DMF.
Jared
--
Jared Head at the Department of Biochemistry, University of Bristol
"A computer lets you make more mistakes faster than any invention in human
history - with the possible exceptions of handguns and tequila."
Mitch Ratliffe
