Immunoprecipitation under reducing conditions and in SDS

S.D. Wainwright ogsdw at ssa.bris.ac.uk
Mon Mar 10 08:58:37 EST 1997

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Dear all 

Does anyone have a method for immunoprecipitation of membrane proteins
which have been solubilised in SDS and reduced with either DTT or 2-ME.

My mAb will only react on immunoblots when the membrane proteins have been
solubilised in the presence of 2-ME.


Thanks for the help 

Shane Wainwright

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