In article <199703271238.MAA27981 at holyrood.ed.ac.uk>,
xxzang at HOLYROOD.ED.AC.UK (Xing-Xing Zang) wrote:
> Dear all
>> Dose anyone know the key sequence in FasL which is essential to bind Fas.
> Thanks.
>> X X Zang
Dear X X,
for simplicity I would assume it is somewhere close to the point where
the point mutation in the gld allel of FasL occured.
It should be possible to find out about that one by searching medline, e.g.:
@C Takahashi, T.//Tanaka, M.//Brannan, C. I.//Jenkins, N. A.//Copeland, N.
G.//Suda, T.//Nagata, S. Generalized lymphoproliferative disease
in mice, caused by a point mutation in the Fas ligand Cell
1994 76 6 969-76 Mar 25 Mice homozygous for lpr
(lymphoproliferation) or gld (generalized lymphoproliferative disease)
develop lymphadenopathy and suffer from autoimmune disease. The lpr mice
have a mutation in a cell-surface protein, Fas, that mediates apoptosis.
Fas ligand (FasL) is a tumor necrosis factor (TNF)-related type II
membrane protein and binds to Fas. Here, mouse Fasl gene was isolated and
localized to the gld region of mouse chromosome 1. Activated splenocytes
from gld mice express Fasl mRNA. However, FasL in gld mice carries a point
mutation in the C-terminal region, which is highly conserved among members
of the TNF family. The recombinant gld FasL expressed in COS cells could
not induce apoptosis in cells expressing Fas. These results indicate that
lpr and gld are mutations in Fas and Fasl, respectively, and suggest
important roles of the Fas system in development of T cells as well as
cytotoxic T lymphocyte-mediated cytotoxicity.
jorg
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