IUBio

isotype controls and Fc receptors

Christie Blackman cfb7 at psu.edu
Thu May 1 15:43:56 EST 1997


I was hoping someone could explain to me what exactly is an "isotype control" 
for immunofluorescence assays?

Also, can anyone give me the story on Fc receptors?  I work with both THP-1 
cells (human macrophage like) and J774 (murine).  Any good literature?  Ideas 
as to how many receptors/cell?
Thanks in advance to anyone who can help!

Melissa Callahan




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