In article <01bc588a$9ae71d60$060b258a at rhgf001>,
N. Sheikh <rhgf001 at mailrelay.qmw.ac.uk> wrote:
>Just wanted to know if anybody can help out on CTLs: am immunising mice
>(C57bl - H-2Kb) with OVA/adjuvants and assaying for CTL activity using a
Immunizing with whole protein is a very inefficient way of stimulating
CTL, even with adjuvants. If you want OVA-specific CTL, you might want to
try a virus vector, or if you're more ambitious you can try ovalbumin
linked to polystyrene beads or ovalbumin peptides as described in some of
the papers from Ken Rock's lab.
Ian
--
Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
"-but as he was a York, I am rather inclined to suppose him a
very respectable Man." -Jane Austen, The History of England
