IUBio

isotype controls and Fc receptors

Nithianandan Selliah thamilan at worldnet.att.net
Sun May 4 23:54:54 EST 1997


Christie Blackman wrote:
> 
> I was hoping someone could explain to me what exactly is an "isotype control"
> for immunofluorescence assays?
> 
> Also, can anyone give me the story on Fc receptors?  I work with both THP-1
> cells (human macrophage like) and J774 (murine).  Any good literature?  Ideas
> as to how many receptors/cell?
> Thanks in advance to anyone who can help!
> 
> Melissa Callahan

Hi,
Isotype control is the isotype of the primary antibody you are using for
the experiment. 
Nithi



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