Christie Blackman wrote:
>> I was hoping someone could explain to me what exactly is an "isotype control"
> for immunofluorescence assays?
>> Also, can anyone give me the story on Fc receptors? I work with both THP-1
> cells (human macrophage like) and J774 (murine). Any good literature? Ideas
> as to how many receptors/cell?
> Thanks in advance to anyone who can help!
>> Melissa Callahan
Hi,
Isotype control is the isotype of the primary antibody you are using for
the experiment.
Nithi