To fellow scientists,
I am having trouble isolating my protein of interest from a crude plant
extract. I am isolating the antibody-antigen complex by using Protein A
bound to sepharose. I believe the problem may be that the conditions for
antibody - antigen binding are incorrect. Does anyone know what are the
ideal conditions for immunoprecipitation. Perhaps someone has a good
protocol for immunoprecipitating plant proteins.
My crude extract is suspended in 0.1M Tris (pH 8), 3mM EDTA, 3mM DTT,
0.5M sucrose, 0.05% Triton.
Thanks in advance,
Mike Mason
M.Mason at Botany.uq.edu.au
