I've been working with an antibody titled:
28-8-6S
(ATCC designation:HB-51)
This antibody recognizes H-2Kb or Db molecules and has no known (at
least published) cross-reactivity to other haplotypes.
I used this antibody for a FACS experiment in an attempt to
discriminate P815 cells (H2d) from EL4 cells (H2b). Both of these cell
lines are transfected with an A2.1/Kb chimaeric molecule containing the
alpha-1/2 domains of HLA A2.1 and the alpha3 domain of Kb. I find that
the antibody stains *both* cell lines. At first I was puzzled. Then, I
realized that if the 28-8-6S antibody recognizes the alpha3 domain of
Kb, that could explain its recognition of my transfected P815 cells.
My question: Does anyone know the specificity of the 28-8-6S antibody?
I will probably use a different antibody in future experiments (i.e.
Db-restricted), but would still like to know the answer to this
question.
Thank-you.
28-8-6S (Anti H-2KbDb)
--
Cheers,
Randy
friedr at med.unc.edu " I am Homer of Borg.
(.sig stolen from someone) Resistance
is..........Mmmmmm..donuts!!"
