IUBio

Antibody binding

kklimpel at biopraxis.com kklimpel at biopraxis.com
Fri May 9 18:37:49 EST 1997


How tightly will an antibody hold onto an antigen?  If you have 100 ab 
(with two binding sites) of a realatively high affinity (10E-9) in a 
liter and 200 antigen molecules, will they all be bound by the ab, or 
will the off rate be such that only a percentage will be bound or will 
none bind because the solution is to dilute?  I'm  looking for an 
explanation of antibody affinity that I can relate to in simple terms. 
Any help, guesses would be appreciated.    

Thanks,

-Kurt



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