How tightly will an antibody hold onto an antigen? If you have 100 ab
(with two binding sites) of a realatively high affinity (10E-9) in a
liter and 200 antigen molecules, will they all be bound by the ab, or
will the off rate be such that only a percentage will be bound or will
none bind because the solution is to dilute? I'm looking for an
explanation of antibody affinity that I can relate to in simple terms.
Any help, guesses would be appreciated.
Thanks,
-Kurt