I am a clinician with an interest in using lymphocyte proliferation
testing to quantify cell mediated immune responses and identify T cell
epitopes. I recently had a conversation with a grad student in
immunology and he brought up several points that seemed to
controversial among his mentors. First of all, is lymphocyte
proliferation testing still a well accepted test to look at cell
mediated responses? Is priming with antigen or Il-2 for a week prior
to actual testing a common modification of the "traditional"
lymphocyte proliferation testing? Are the data generated (either
stimulation indices or counts) normally distrubuted? Are
non-radioactive methods a well accepted alternative to radiolabeled
thymidine?
You can either e-mail me or post to the group. Thanks -
Kevin Ault, MD University of Iowa Hospitals and Clinics
Iowa City IA USA kevin-ault at uiowa.edu
