Tania, Humphrey, t.humphrey at botany.uq.edu.au wrote:
>> I have a 6.5kDa protein I plan injecting into rabbits for raising antibodies.
> Is this large enough on its own for successful antibody production or would I
> be better off producing it as a fusion protein to increase the size?
>> Tania Humphrey
>> Plant Genetic Engineering Laboratory
> Department of Botany
> University of Queensland Australia
>t.humphrey at botany.uq.edu.au
Sure, that should be large enough. The minimum is usually 50-60
residues for peptides. I think it will work fine (but of course, you'll
never know until you do it :-). What is your adjuvant? Remember,
mammals have adverse reactions to oil-based adjuvants. If you're going
to sacrifice the bunny after getting your Ab, then you'll be fine using
Freund's. Check with your university's Animal Care Committee to find
out what guidlines (restrictions) they have.
--
Tony Schountz
tonys at utkux.utk.edu
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