IUBio

How do I detect for plasminogen activity?

Grace Ho EHO17 at alpha2.curtin.edu.au
Fri May 23 03:41:01 EST 1997


Hello fellow immunologist,
								I think I may be posting this email on the wrong newsgroup but I 
thought of just giving it a try...
								I am trying to measure the activation of plaminogen by a 
specific protein with the help of tPA (tissue plasminogen activator)....  
I have heard of the method where you use chromogenic substrate such as 
Leu-Lys-p-nitroaniline....  But the reagent is pretty hard to obtain.... 
Is there a better way to measure this or I have to resolve in tracking 
this particular chromogenic substrate down...????
							Thanks!!!!



Your sincerely,
Grace Ho (Bsc) (Hons)
Curtin University of Technology
Western Australia
Email:  EHO17 at alpha1.curtin.edu.au



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