In article <33894AD7.25E1 at alpha2.curtin.edu.au>, Grace Ho
<EHO17 at alpha2.curtin.edu.au> wrote:
> Hello everyone,
> I am Grace and I am currently
undertaking my PhD studies in
> immunology. And I have a question for you all... I know I may be asking
> a really silly question but I have tried everything and I am not
> satisfied with the results....
> You see, I am currently working
with a highly insoluble recombinant
> protein.. (I mean highly insoluble in water!!) Anyway, I have to put my
> protein, once solubilised into my cell culture media to test for cells
> stimulation in response to the protein.... Therefore the solvent for my
> recombinant protein has to be pretty gentle to the cells... I just
> can't seem to find anything that can fulfill this particular criteria..
> Can any please spare me some thoughts....!!!! It is all much
> appreciated!!!! Thank you !!!
>>>> Sincerely,
> Grace Ho BSc (Hons)
> Curtin University of Technology
> Western Australia
> Email: EHO17 at alpha1.curtin.edu.au
You may use DMSO or 100% Ethanol to solubilise your protein. You should
make a very high concentration e.g. 100X of your effective dosage for
applying into culture then the toxic effect of the solvent may be
minimised. Ultrasound may help your too.
Good luck!
--
Francis Chun-wai CHAN
Dept of Ortho. & Trauma,
CUHK
cwaichan at cuhk.edu.hk
