Grace Ho <EHO17 at alpha2.curtin.edu.au> éCRIVAIT (wrote) :
> You see, I am currently working with a highly insoluble recombinant
> protein.. (I mean highly insoluble in water!!) Anyway, I have to put my
> protein, once solubilised into my cell culture media to test for cells
> stimulation in response to the protein.... Therefore the solvent for my
> recombinant protein has to be pretty gentle to the cells...
Try Guanidium chloride 5M or 7M in the buffer of your choice.
You should solubilize your protein in a small volume.
To assay it, just dilute it out (GuCl as control).
It worked for IL-2, IL-1, gamma-IFN, etc.
Why not your stuff ?
__ __
______________________________ _____ / \/ \
jpg at pasteur.fr _____ __o | |
Ideal doctors are patient ------ -\<, \ /
------------------------------ -------- O' O \ /
"Idéalement, médecins sont patients" traduisit-il approximativement\/
"Et les miens le sont-ils ?" répliqua-t-elle du tac au tac
Ah ! le français (la française) et les trésors de 'sa' langue.
