In article <338E908D.C9E at alpha2.curtin.edu.au> Grace Ho <EHO17 at alpha2.curtin.edu.au> writes:
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>From: Grace Ho <EHO17 at alpha2.curtin.edu.au>
>Newsgroups: bionet.immunology
>Subject: Route of immunisation????
>Date: Fri, 30 May 1997 16:32:13 +0800
>Organization: Curtin University of Technology
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>Hello everyone,
> I am pretty confuse here about the route of immunisation!!! I am
>currently working with a mouse model where I take my protein antigen in
>Freund's adjuvant.... and immunised the victim via intraperitoneal route
>.... in the hope of stimulating a good systemic response as well as good
>T-cells reponse....
> However, I have been receiving advice from people (immunologists)
>and found that ip. immunisation is not a good way to go about if I want
>a good systemic/T-cells reponse..... They all recommended subcutaneous
>injection or intradermal injection....
> Problem is I am so used to ip. route immunisation and my work have
>been sort of based on ip. immunisation.... and things have not been
>working very well for me... and on the other hand, if I change the route
>of immunisation.. then my work is sort of down the drain!!!!
> Does anyone have any insight to this!!!!????? Please!!!??
>Thank you!!!!
>Sincerely,
>Grace Ho PhD student
>Curtin University of Technology
>Western Australia
>Email: EHO17 at alpha1.curtin.edu.au
If the only reason you have to continue with i.p. immunization is that you
are used to it, maybe you should review your entire approach to research.
Omar O. Barriga
