IUBio

immunofluorescence

mark mark.haynes at mail.tju.edu
Thu Nov 6 16:46:47 EST 1997


Chunming Dong wrote:
> 
> Hello there:
> 
> I am reviewing a grant.  The investigator proposed to use
> immunofluorescence (IF) staining on paraffin-embedded tissue sections.
> Hematoxylin counterstaining will be used to view the nuclei.  I just
> wonder whether IF can be used on paraffin sections or not and whether
> fluorescence microscope will be able able to see the nuclei
> counterstained by hematoxylin or not.  Any opinions will highly
> appreciated.
> 
> Chunming

I'm curious. What type of grant are you reviewing (NIH, NSF, National 
foundation, private group)?  Also, is it in your field of expertise?  DO 
you have a lab with students?  Is there a library near your lab?

Mark H.



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