In message <2A2AD7F5E19 at prl.pulmonary.ubc.ca> - CDong at PRL.PULMONARY.UBC.CA ("Chunming Dong") writes:
:>:>Hello there:
:>:>I am reviewing a grant. The investigator proposed to use
:>immunofluorescence (IF) staining on paraffin-embedded tissue sections.
:>Hematoxylin counterstaining will be used to view the nuclei. I just
:>wonder whether IF can be used on paraffin sections or not and whether
:>fluorescence microscope will be able able to see the nuclei
:>counterstained by hematoxylin or not. Any opinions will highly
:>appreciated.
:>:>Chunming
This works beautifully. It requires that the primary antibodies to detect the antigen are selected
for paraffin sections. You may combine this technique with a variety of other detection methods on
tissue sections. All you need is a person dedicated to sophisticated experiments
All the best
Bernhard Kleine
(bernhard.kleine at medizin.uni-ulm.de)