IUBio

immunofluorescence

Dr. Bernhard Kleine bernhard.kleine at medizin.uni-ulm.de
Wed Nov 12 02:29:33 EST 1997


In message <2A2AD7F5E19 at prl.pulmonary.ubc.ca> - CDong at PRL.PULMONARY.UBC.CA ("Chunming Dong") writes:
:>
:>Hello there:
:>
:>I am reviewing a grant.  The investigator proposed to use 
:>immunofluorescence (IF) staining on paraffin-embedded tissue sections. 
:>Hematoxylin counterstaining will be used to view the nuclei.  I just 
:>wonder whether IF can be used on paraffin sections or not and whether
:>fluorescence microscope will be able able to see the nuclei 
:>counterstained by hematoxylin or not.  Any opinions will highly 
:>appreciated.
:>
:>Chunming

This works beautifully. It requires that the primary antibodies to detect the antigen are selected
for paraffin sections. You may combine this technique with a variety of other detection methods on
tissue sections. All you need is a person dedicated to sophisticated experiments

All the best

Bernhard Kleine
(bernhard.kleine at medizin.uni-ulm.de)




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