> I'm looking for murine anti-TNF and anti IL-1 Abs, to use on cryostat cut
> sections of mouse lung tissue. Although such Abs exist for FACS analysis
> and ELISAs I have have not been able to find any specifically for
> immuno-histochemistry work.
> snip<
> 1) Can I use Abs used for ELISA/FACS on tissue sections ?
> 2) Does anyone know where to get above mentioned Abs for histological work.
HI---alot of times ab suppliers are not conducting the research. If they
know of a reference that used the antibody for histo then they will ref
the paper + say the ab is okay. I would purchase or if possible, get an
aliquot and test it for histo with standard checkerboard analysis. I
have used human anti-TNF with cryo-sections and have reasonable results.
these abs were from genzyme and R&D
