I am trying to isolate monocytes from human PBMC by adherence. When I
use RPMI-10% FCS, yield is extremely poor. When I use RPMI with no
sera, viability is low. What media and what plates should I use? What
is the expected viability?
Thanks,
David Yu
--
35-40 Rehabilitation Center, Rheumatology Division, UCLA, 1000 Veteran
Ave., Los Angeles, CA90095-167022, e-mail: dtyyu at ucla.edu, FAX:
(310)-206-8606.
