sunkei wrote:
>> Dear netters:
> I encountered a problem and need help from you. I have cloned a 800bp
> fragment into the pIG vector, which could expressed an fusion protein with
> Fc fragment of IgG1 at the end of the target protein. The expressed fusion
> protein was identified by affinity chromatography and sandwich ELSIA. This
> fusion protein is not stable, after I purified this protein by affinity
> chromatography,I keep it in PBS at -20c. But its half span under this
> condition is about 14 days. So, my question is how to keep my protein
> stable. The aim of my experiment is to do immunohistochemistry using this
> protein, If adding BSA would confluence my results.
>> thank you in advance.
>> Sun Kai, Ph.D.
> Department of Immunology
> The Fourth Military Medical University
> Xi'an, 710032
> PR China
>> e-mail: sunkei at pub.xa-online.net.cn
Due to instability of purified fusionproteins in solution, I know always
freecedry my preperations and thereby the halflife is nearly indefinite
;-)
--
************************************************************************
If you are are not part of the solution, you are part of the precipitate
************************************************************************
Per Mygind, Cand.scient
Department of Medical Microbiology and Immunology
The Bartholin Building
University of Aarhus
Denmark
phone : 89 42 17 47
fax :