IUBio

how to keep protein stable

Per Mygind perm at medmicro.aau.dk
Sun Jan 4 11:21:31 EST 1998


sunkei wrote:
> 
> Dear netters:
>         I encountered a problem and need help from you. I have cloned a 800bp
> fragment into the pIG vector, which could expressed an fusion protein with
> Fc fragment of IgG1 at the end of the target protein. The expressed fusion
> protein was identified by affinity chromatography and sandwich ELSIA. This
> fusion protein is not stable, after I purified this protein by affinity
> chromatography,I keep it in PBS at -20c. But its half span under this
> condition is about 14 days. So, my question is how to keep my protein
> stable. The aim of my experiment is to do immunohistochemistry using this
> protein, If adding BSA would confluence my results.
> 
> thank you in advance.
> 
> Sun Kai, Ph.D.
> Department of Immunology
> The Fourth Military Medical University
> Xi'an, 710032
> PR China
> 
> e-mail: sunkei at pub.xa-online.net.cn

Due to instability of purified fusionproteins in solution, I know always
freecedry my preperations and thereby the halflife is nearly indefinite
;-) 


-- 
************************************************************************
If you are are not part of the solution, you are part of the precipitate
************************************************************************

Per Mygind, Cand.scient

Department of Medical Microbiology and Immunology
The Bartholin Building
University of Aarhus
Denmark
phone : 89 42 17 47
fax   :



More information about the Immuno mailing list

Send comments to us at biosci-help [At] net.bio.net